Hames, Kazanna C., Coen, Paul M., King, Wendy C., Anthony, Steven J., Stefanovic-Racic, Maja, Toledo, Frederico G. S., Lowery, Jolene B., Helbling, Nicole L., Dube, John J., DeLany, James P., Jakicic, John M., Goodpaster, Bret H.
funding text
This study was supported by the University of Pittsburgh CTRC (M01RR00056), the Obesity and Nutrition Research Center (1P30DK46204), and a grant from the Commonwealth of Pennsylvania Department of Health.
abstract
ObjectiveTo determine effects of physical activity (PA) with diet-induced weight loss on energy metabolism in adults with severe obesity. MethodsAdults with severe obesity (n = 11) were studied across 6 months of intervention, then compared with controls with less severe obesity (n = 7) or normal weight (n = 9). Indirect calorimetry measured energy metabolism during exercise and rest. Markers of muscle oxidation were determined by immunohistochemistry. Data were presented as medians. ResultsThe intervention induced 7% weight loss (P = 0.001) and increased vigorous PA by 24 min/wk (P = 0.02). During exercise, energy expenditure decreased, efficiency increased (P 0.03), and fatty acid oxidation (FAO) did not change. Succinate dehydrogenase increased (P = 0.001), but fiber type remained the same. Post-intervention subjects' resting metabolism remained similar to controls. Efficiency was lower in post-intervention subjects compared with normal-weight controls exercising at 25 W (P 0.002) and compared with all controls exercising at 60% VO2peak (P 0.019). Resting and exercise FAO of post-intervention subjects remained similar to adults with less severe obesity. Succinate dehydrogenase and fiber type were similar across all body weight statuses. ConclusionsWhile metabolic adaptations to PA during weight loss occur in adults with severe obesity, FAO does not change. Resulting FAO during rest and exercise remains similar to adults with less severe obesity.