Pancreas-enriched miRNAs are altered in the circulation of subjects with diabetes: a pilot cross-sectional study

Seyhan, Attila A., Lopez, Yury O. Nunez, Xie, Hui, Yi, Fanchao, Mathews, Clayton, Pasarica, Magdalena, Pratley, Richard E.

The authors would like to thank Quilin Mars for proof reading the manuscript. This study was funded by the Florida Hospital's Translational Research Institute for Metabolism and Diabetes.

The clinical presentation of diabetes sometimes overlaps, contributing to ambiguity in the diagnosis. Thus, circulating pancreatic islet-enriched microRNAs (miRNAs) might be useful biomarkers of beta-cell injury/dysfunction that would allow more accurate subtyping of diabetes. We measured plasma levels of selected miRNAs in subjects with prediabetes (n = 12), type 2 diabetes (T2D, n = 31), latent autoimmune diabetes of adults (LADA, n = 6) and type 1 diabetes (T1D, n = 16) and compared them to levels in healthy control subjects (n = 27). The study was conducted at the Translational Research Institute for Metabolism and Diabetes (TRI-MD), Florida Hospital. MiRNAs including miR-375 (linked to beta-cell injury), miR-21 (associated with islet inflammation), miR-24.1, miR-30d, miR-34a, miR-126, miR-146, and miR-148a were significantly elevated in subjects with various forms of diabetes compared to healthy controls. Levels of several miRNAs were significantly correlated with glucose responses during oral glucose tolerance testing, HbA(1c), beta-cell function, and insulin resistance in healthy controls, prediabetes, and T2D. These data suggest that miRNAs linked to beta-cell injury and islet inflammation might be useful biomarkers to distinguish between subtypes of diabetes. This information could be used to predict progression of the disease, guide selection of optimal therapy and monitor responses to interventions, thus improving outcomes in patients with diabetes.

Pancreas-enriched miRNAs are altered in the circulation of subjects with diabetes: a pilot cross-sectional study Article